ELISA Substrates and Reagents
ABclonal Technology Phospho-AKT1-S473 Rabbit pAb
This gene encodes a member of the AKT serine-threonine kinase family, involved in regulating cell proliferation, survival, metabolism, and angiogenesis. AKT proteins are activated by phosphorylation via phosphoinositide 3-kinase (PI3K) and play key roles in various signaling pathways, including those involving receptor tyrosine kinases and G-protein coupled receptors. AKT activation requires phosphorylation of threonine 308 and serine 473. The PI3K/AKT pathway is crucial for tumor cell survival by inhibiting apoptosis and activating survival factors. AKT also interacts with the mTOR pathway, affecting translation initiation and is often dysregulated in cancers. Mutations in this gene are linked to various cancers, Proteus syndrome, Cowden syndrome, and other growth disorders. Multiple transcript variants exist due to alternative splicing.
ABclonal Technology Phospho-IκBα-S36 Rabbit pAb
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
ABclonal Technology Phospho-RELB-S573 Rabbit pAb
Enables RNA polymerase II cis-regulatory region sequence-specific DNA binding activity and protein kinase binding activity. Involved in lymphocyte differentiation and negative regulation of interferon-beta production. Located in cytosol and nucleoplasm. Part of chromatin, nucleus, and transcription repressor complex. Colocalizes with centrosome. Implicated in breast cancer and immunodeficiency 53. Biomarker of breast cancer and transitional cell carcinoma.
ABclonal Technology Phospho-STK3/MST2-T117 Rabbit pAb
This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
ABclonal Technology Phospho-SnRK2.6-S175 pAb
Activator of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomata closure in response to drought, darkness, high CO2, plant pathogens, or decreases in atmospheric relative humidity (RH) (PubMed30361234).
American Research Products Inc Mouse urokinase plasminogen ac
Mouse urokinase plasminogen actiVator uPA ELISA kit from CUSABIO Detection Range 15 6 pg/mL-1000 pg/mL Sensitivity 3 9 pg/mL Sample serum plasma tissue homogenates Method Sandwich
Neogen Diagnostics Custom TMB Substrate, 200 ML, HRP, Microwell, Colorimetric
Custom TMB Substrate is a one bottle stabilized chromogenic substrate for use with horseradish peroxidase (HRP) immunoassays. It contains both 3,3,5,5 tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2). Custom TMB Substrate develops a deep blue color in the presence of peroxidase labelled conjugate. This substrate has been optimized for use with microwell assays, especially ELISA. Custom TMB is not for use with assays requiring a precipitating substrate. Stable for a minimum of 36-months when stored at 2-8C.
Meso Scale Discovery RPLEX HUM Fcepsilon RII ASYQP
The Meso Scale Discovery FC-Epsilon-RII Assay Kit is a highly sensitive immunoassay that quantitatively measures the soluble form of the Epsilon RII receptor in biological samples such as serum plasma and cell culture supernatants This assay utilizes MSD s proprietary electrochemiluminescent detection technology and provides a rapid 3 5 hour protocol from sample to results The FC-Epsilon-RII kit includes a 96-well plate coated with a mouse anti-human FC-Epsilon-RII capture antibody a ruthenylated mouse anti-human FC-Epsilon-RII detection antibody calibrators and an MSD SECTOR Imager for readout The assay has a sensitivity of 19 pg/mL and a broad dynamic range up to 10 000 pg/mL
ABclonal Technology Phospho-MARCKS-S162 Rabbit pAb
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
ABclonal Technology Phospho-GSK3α-S21 Rabbit pAb
This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimers disease.
American Research Products Inc Rat heat shock protein 27 HSP
Rat heat shock protein 27 HSP-27 ELISA Kit from CUSABIO Detection Range 0 78 ng/mL-50 ng/mL Sensitivity 0 195 ng/mL Sample serum plasma tissue homogenates Method Sandwich
Cayman Chemical TMB 8hydrochlorIde 10mg
A non-competitive antagonist of nAChRs (IC50s = 390 and 350 nM, respectively, for human muscle-type and and α3β4 subunit-containing ganglionic nAChRs); an inhibitor of intracellular calcium availability in smooth and skeletal muscle; blocks the contractile response in isolated rabbit aortic strip when used at a concentration of 50 µM; inhibits calcium influx and efflux in isolated guinea pig ileum when used at a concentration of 65 µM; used in the study of intracellular calcium dynamics; dose-dependently inhibits PKC activity
ABclonal Technology Phospho-HSP27/HSPB1-S78 Rabbit pAb
This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.
Genscript Corporation CEACAM-5/CD66eBiotin His
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated